Description |
Product Name: Fibroblast Growth Factor-Basic Mouse Recombinant Synonyms: HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b. Description: Fibroblast Growth Factor-basic Mouse Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.3kDa. The FGF-2 is purified by proprietary chromatographic techniques. Uniprot Accesion Number: P15655 Amino Acid Sequence:MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS. Source: Escherichia Coli. Physical Appearance and Stability: Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FGF-basic should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Formulation and Purity: FGF-b was lyophilized from 5mM Na2PO4, pH7.5 and 50mM NaCl. Greater than 95.0% as determined by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor b in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The activity as calculated by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 1ng/ml corresponding to a specific activity of 1, 000, 000 units/mg. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |