Insulin Human Recombinant, Yeast

Lyophilized powder at room temperature.

Description: Insulin Human Recombinant produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Zinc content was found to be 0.4%. Insulin is purified by proprietary chromatographic techniques.
Source: Saccharomyces cerevisiae.
Physical Appearance and Stability: Lyophilized Insulin although stable at room temperature, should be stored at 4C. Upon reconstitution Insulin should be stored at -20C to -80C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from 50mM Sodium Chloride solution. Greater than 95.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Insulin in PBS pH-7.5.
Biological Activity: 27.5 units/mg. Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts). The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator.