Macrophage Migration Inhibitory Factor Human, Recombinant His Tag N-Terminus

Lyophilized powder at room temperature.

MIF human Recombinant, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated, polypeptide chain having amino acids from 1-114 and having a molecular mass of 16.6 kDa.
Amino Acid Sequence:MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA.
Physical Appearance and Stability: Liquid MIF although stable 4C for 1 week, should be stored below -18C. Please prevent freeze-thaw cycles.
Formulation and Purity: 1mg/ml solution containing PBS pH-7.4. Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.