Macrophage Migration Inhibitory Factor Mouse Recombinant

Lyophilized powder at room temperature.

MIF Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 12.5kDa.The MIF is purified by proprietary chromatographic techniques.
Amino Acid Sequence:MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTNDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA.
Physical Appearance and Stability: Lyophilized MIF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution MIF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: Lyophilized from a 0.2um filtered concentrated solution in 1xPBS, pH 7.4 and 5% trehalose. Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized MIF in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.