beta Nerve Growth Factor Human Recombinant, HEK

Lyophilized powder at room temperature.

Beta-NGF Human Recombinant produced in HEK cells is a non-glycosylated non-disulfide linked homodimer, having a total molecular weight of 13kDa.The b-NGF is purified by proprietary chromatographic techniques.
Physical Appearance and Stability: Lyophilized b-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution b-NGF should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The b-NGF was lyophilized from 1mg/ml in 1xPBS. Greater than 95% as obsereved by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized b-NGF in sterile 1xPBS containing 0.1% endotoxin-free recombinant HSA.
Biological Activity: The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line), the ED50 is < 4ng/ml.