Beta Nerve Growth Factor Human Recombinant

Lyophilized powder at room temperature.

Nerve Growth Factor-beta Human Recombinant produced in E.Coli is a non-covalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical 121 amino acids with a molecular weight of two 13.6 kDa polypeptide monomers.The NGF-b is purified by proprietary chromatographic techniques.
Amino Acid Sequence:MSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA.
Physical Appearance and Stability: Lyophilized Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution NGF-Beta should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The beta-NGF protein was lyophilized from a 0.2um filtered solution containing 0.1% Trifluoroacetic Acid (TFA). Greater than 95.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized NGF-b in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50, calculated by its ability to stimulate proliferation of TF-1 cells and is typically < 1.0 ng/ml, corresponding to a specific activity of > 1, 000, 000units/mg.