Calbindin-1 Rat Recombinant

Lyophilized powder at room temperature.

Recombinant Rat Calbindin-1 produced in E.Coli.The Rat CALB1 is purified by proprietary chromatographic techniques.
Physical Appearance and Stability: Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CABP28K should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein was lyophilized from a concentrated solution (1mg/ml) containing 50mM NaHCO3. Greater than 90% as determined by SDS-PAGE.
Application: CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedureA- Dilute 1 ul of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).B- Add 1 ug of the recombinant protein to 1 ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.
Solubility: It is recommended to reconstitute the lyophilized CABP28K in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.