S100 Calcium Binding Protein A1 Human Recombinant

Lyophilized powder at room temperature.

S100A1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 103 amino acids which include a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 11.66 kDa. S100A1 Human Recombinant is purified by proprietary chromatographic techniques.
Amino Acid Sequence:MKHHHHHHAS GSELETAMET LINVFHAHSG KEGDKYKLSK KELKELLQTE LSGFLDAQKD VDAVDKVMKE LDENGDGEVD FQEYVVLVAA LTVACNNFFW ENS.
Physical Appearance and Stability: Lyophilized S100A1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution S100A1 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The S100A1 protein was lyophilized from 0.4um filtered solution at a concentration of 0.5mg/ml containing 20mM Tris pH-7.5 and 50mM NaCl. Greater than 95.0% as determined by SDS-PAGE.
Solubility: Add deionized water to a working concentration approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.