Chromatin Modifying Protein 4A Human Recombinant

Lyophilized powder at room temperature.

CHMP4A Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (1-265 and having a molecular mass of 32.0kDa.CHMP4A is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSRRRPEDGL GKAGPCVMRH HPPRSKAEVW RTLRGGGGRG ELAMSGLGRL FGKGKKEKGP TPEEAIQKLK ETEKILIKKQ EFLEQKIQQE LQTAKKYGTK NKRAALQALR RKKRFEQQLA QTDGTLSTLE FQREAIENAT TNAEVLRTME LAAQSMKKAY QDMDIDKVDE LMTDITEQQE VAQQISDAIS RPMGFGDDVD EDELLEELEE LEQEELAQEL LNVGDKEEEP SVKLPSVPST HLPAGPAPKV DEDEEALKQL AEWVS
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: The CHMP4A solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 0.1mMPMSF, 1mM EDTA, 2mM DTT and 50% glycerol. Greater than 90% as determined by SDS-PAGE.