Fibronectin Type III Domain Containing 5 Human Recombinant, Yeast

Lyophilized powder at room temperature

Fibronectin Type III Domain Containing 5 Human Recombinant produced in yeast is a glycosylated, polypeptide chain containing 110 amino acids and having a molecular mass of 20-25 kDa.FNDC5 is purified by proprietary chromatographic techniques.
Amino Acid Sequence:S_P__S__A__P__V__N__V__T__V__R__H__L__K__A__N__S__A__V__V__S_
W__D__V__L__E__D__E__V__V__I__G__F__A__I__S__Q__Q__K__K__D _V__R__M__L__R__F__I__Q__E__V__N__T__T__T__R__S__C__A__L__W_
_ D__L__E__E__D__T__E__Y__I__V__H__V__Q__A__I__S__I__Q__G__Q_
S__P__A__S__E__P__V__L__F__K__T__P__R__E__A__E__K__M__A__S_ K__N__K__D__E__V__T__M__K__E.
Source: Yeast.
Physical Appearance and Stability: Lyophilized Fibronectin Type III Domain Containing 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FNDC5 should be stored at 4C between 2-7 days and for future use below -18C.Please prevent freeze-thaw cycles.
Formulation and Purity: The FNDC5 was lyophilized from 0.45 um filtered solution in PBS. Greater than 98.0% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Fibronectin Type III Domain Containing 5 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The biological activity of recombinant human irisin was measured by the ability to induce UCP-1 expression of adipocytes. The specific activity is 10ng/ml.