Myosin Light Chain 4 Human Recombinant

Lyophilized powder at room temperature.

MYL4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 205 amino acids (1-197 a.a.) and having a molecular mass of 22.6kDa.MYL4 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Amino Acid Sequence:MAPKKPEPKK EAAKPAPAPA PAPAPAPAPA PEAPKEPAFD PKSVKIDFTA DQIEEFKEAF SLFDRTPTGE MKITYGQCGD VLRALGQNPT NAEVLRVLGK PKPEEMNVKM LDFETFLPIL QHISRNKEQG TYEDFVEGLR VFDKESNGTV MGAELRHVLA TLGEKMTEAE VEQLLAGQED ANGCINYEAF VKHIMSGLEH HHHHH.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: MYL4 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 150mM NaCl, 1mM DTT and 20% glycerol. Greater than 90% as determined by SDS-PAGE.