SRY (sex determining region Y)-box 2 Human Recombinant, TAT

Lyophilized powder at room temperature.

Sox2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 330 amino acids (317 aa residues of the full length Sox2) and having a molecular mass of 36kDa.Sox2 is fused to a 13 amino acid TAT peptide at C-terminus (GGYGRKKRRQRRR) & purified by proprietary chromatographic techniques.
Amino Acid Sequence:MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHMGGY GRKKRRQRRR.
Physical Appearance and Stability: Lyophilized Sox2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Sox2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: Lyophilized from a 0.2um filtered concentrated solution in 2xPBS, pH 7.4 and 5% trehalose. Greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Sox2 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.