Thioredoxin E.Coli Recombinant

Lyophilized powder at room temperature.

Recombinant Thioredoxin was purified from E. coli harboring its gene.
Amino Acid Sequence:HMSDKIIHL TDDSFDTDVLKADGAIL VDFW AEWCGPCKMIAPILDEI GKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGAL DANLA.
Physical Appearance and Stability: TRX although stable at 4C for 3 weeks, should be stored desiccated below -18C. Please prevent freeze thaw cycles.
Formulation and Purity: Each mg of protein contains 20mM phosphate buffer pH 7.4. Greater than 90.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized TRX in sterile 18MO-cm H2O.
Biological Activity: TRX activity is assayed by measuring the change in absorbance at 650 nm at 25C using 0.13uM bovine insulin containing 0.33mM DTT (pH 6.5).The specific activity was found to be 3IU/mg.