Ubiquitin Carboxyl-Terminal Hydrolase L1 Human Recombinant

Lyophilized powder at room temperature.

UCHL1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 223 amino acids (1-223 a.a.) and having a molecular mass of 24.8 kDa (molecular weight on SDS-PAGE will appear higher).
Amino Acid Sequence:MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: The UCHL1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 2mM EDTA. Greater than 95.0% as determined by SDS-PAGE.
Biological Activity: Specific activity: > 300 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.