HIV-1 nef Recombinant

Lyophilized powder at room temperature.

Description: The E.coli derived 20kDa recombinant protein genotype IIIB is a non-glycosylated polypeptide chain, containing the HIV-1 nef immunodominant regions, 3-190 amino acids. The HIV-1 nef is fused to beta-galactosidase (114 kDa) at the N-terminus.
Amino Acid Sequence:kwskssv igwptvrerm rraepaadgv gaasqdlekh gaitssntaa tnadcawleaqeeeevgfpv tpqvplrpmt ykaavdlshf lkekgglegl ihsqrrqdil dlwiyhtqgyfpdwqnytpg pgirypltfg wcyklvpvep ekleeankge ntsllhpvsl hgmddperevlewrfdsrla.
Physical Appearance and Stability: HIV-1 nef although stable at 4C for 1 week, should be stored below -18C. Please prevent freeze thaw cycles.
Formulation and Purity: HIV-1 nef protein solution in 8M urea, 20mM Tris-HCl pH 8.0, 10mM b-mercaptoethanol. Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.
Application: HIV-1 nef antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.