Anti-TPH2 Antibody

Cell Markers, Neuronal Markers, Serotonergic Neuronal Markers

United States

56 kDa

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Tryptophan hydroxylase 2 (TPH2) is an enzyme involved in the biosynthesis of serotonin, a neurotransmitter that plays a crucial role in regulating mood, appetite, and sleep. TPH2 specifically catalyzes the conversion of tryptophan to 5-hydroxytryptophan (5-HTP), a precursor to serotonin. Unlike its counterpart TPH1, which is primarily found in the periphery, TPH2 is predominantly expressed in the central nervous system, particularly in the raphe nuclei of the brainstem. The genetic regulation and expression of TPH2 have been extensively studied in the context of various psychiatric disorders, including depression and anxiety. Research on TPH2 aids in the development of targeted therapeutic interventions focusing on modulating serotonin levels in the brain. Recent studies have shown TPH2 acting in a sex-dependent manner for body processes such as energy balance (Liu et al. Endocrinology, 2021) and anxiety behaviors (Li et al. Neuropharmacology, 2022).

Polyclonal

1:100-1:500

Two synthetic peptides located near the C-terminus of TPH2 (Uniprot - Q8IWU9)

No cross-reactivity reported

12 months from receipt of product

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification