PAX6 (AN2, Aniridia type II protein, Oculorhombin, Paired box gene 6 (aniridia keratitis), Paired box homeotic gene 6, Paired box protein Pax-6, Sey, WAGR)

Antibodies / Antibodies-Transcription Factors

-20°C

Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168851-AF.

Recombinant protein fragment corresponding to aa1-300 at the N-terminal of human PAX6 protein. Cellular Localization: Nuclear

Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters’ anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.

Applications: 
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 0.5-1ug/ml
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas or Cerebellum

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.