VEGF-R2 (CD309,Fetal liver kinase 1 (FLK-1),KDR,Kinase insert domain receptor (a type III receptor tyrosine kinase),KRD1,Ly73,Protein tyrosine kinase receptor FLK1,Protein-tyrosine kinase receptor flk-1,Vascular end

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168889-AF.

Recombinant human VEGF-R2 protein. Cellular Localization: Cell surface

CD309, also known as VEGF-R2, KDR3, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, VEGF-D, and VEGF splice isoforms. Ligation of VEGF-R2 with its ligands results in the receptor dimerization and auto-phosphorylation, stimulating endothelial cell proliferation and migration.

Applications: 
Suitable for use in Immunofluorescence, ELISA, Flow Cytometry, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug/million cells 
Immunofluorescence: 0.5-1ug/ml 
Immunoprecipitation: 0.5-1ug/500ug protein lysate
Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 minutes at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
HUVEC cells. Human lymph nodes and tonsils.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.