Csk (Tyrosine-Protein Kinase CSK, C-Src Kinase, Protein-Tyrosine Kinase CYL, CSK)

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.2% sodium azide, 50% glycerol

Recombinant full length Human Csk.

C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of ~50kDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein).

Applications:
Suitable for use in Western Blot, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.