HINFP (HiNF-P,HINFP,HINFP_HUMAN,Histone H4 Gene specific Protein HiNF P,Histone H4 Transcription Factor,Histone Nuclear Factor P,MBD2 (methyl-CpG-Binding Protein)-interacting zinc Finger Protein,MBD2 interacting zinc Fin

Antibodies / Antibodies-Transcription Factors, Zinc (Ring) Finger

-20°C

Supplied as a liquid PBS,  0.1% sodium azide,  50% glycerol.

Recombinant full length Human HINFP.

HiNF-P is a critial transcription factor which is necessay for E2F-independent activation of the Histone H4 multigene family. HiNF-P associates with conserved H4 cell cycle regulatory sequences in vivo. Antisense inhibition of HiNF-P reduces endogenous Histone H4 gene expression. HiNF-P utilizes NPAT/p220, a substrate of the cyclin E/cyclin-dependent kinase 2 (CDK2) kinase complex, as a crucial coactivator to amplify Histone H4 gene transcription. The biological role of HiNF-P is reflected by impeded cell cycle progression into S phase upon antisense-mediated reduction of HiNF-P levels. Research indicates that HiNF-P is the key link in a linear signaling pathway that is initiated with the growth factor-dependent induction of cyclin E/ CDK2 kinase activity at the restriction point and culminates in the activation of Histone H4 genes through HiNF-P at the G1/S phase transition.

Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.