hnRNP K, phosphorylated (S284) (Heterogeneous Nuclear Ribonucleoprotein K, hnRNP K, Transformation up-Regulated Nuclear Protein, TUNP, HNRNPK, HNRPK)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.

Synthetic phosphopeptide derived from human hnRNP K around the phosphorylation site of Serine 284.

The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.

Applications:
Suitable for use in Western Blot, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunocytochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.