Hox-B4 (Homeobox Protein Hox-B4, Homeobox Protein Hox-2.6, Homeobox Protein Hox-2F, HOX2F, HOXB4)

Antibodies / Antibodies-Transcription Factors, Homeobox (HOX)

-20°C

Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.

Synthetic peptide corresponding to amino acids 100-150 of Human Hox-B4.

The homeobox genes encode a family of transcription factors that regulate development and postnatal tissue homeostasis. Encoded by the HOXB4 gene, the nuclear phosphoprotein HoxB4 plays a key role in regulating the balance between hematopoietic stem cell renewal and differentiation. Hematopoietic expression of HoxB4 is regulated by the binding of USF-1 and USF-2, the binding of which may be favored by cytokines promoting stem cell self-renewal versus differentiation. HoxB4 is dependent on AP-1 expression to induce changes in cellular proliferation and differentiation, which increases the levels of cyclin D1, thereby linking HoxB4 with key elements of the cell cycle machinery. HoxB4 also participates in the downregulation of c-Myc expression. It is expressed in developing hair follicles as well as in K-562 and HL-60 cells.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 500-1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.