mTOR,phosphorylated (S2448) (Serine/threonine-Protein Kinase mTOR,FK506-Binding Protein 12-rapamycin Complex-Associated Protein 1,FKBP12-rapamycin Complex-Associated Protein,Mammalian target of rapamycin,mTOR,Mechan

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.

Synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Serine 2442.

mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.