TAL1 (T-Cell acute lymphocytic Leukemia Protein 1, TAL-1, Class A basic Helix-Loop-Helix Protein 17, bHLHa17, Stem Cell Protein, T-Cell Leukemia/lymphoma Protein 5, TAL1, BHLHA17, SCL, TCL5)

Antibodies / Antibodies-Proteins

-20°C

Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.

Synthetic peptide corresponding to amino acids 80-130 of Human TAL1.

Activation of TAL1 characterizes up to 60% of cases of human T cell acute lymphoblastic leukemia, making it the most frequent gain-of-function mutation observed in this disorder. TAL1 (also designated SCL) is a serine phosphoprotein and basic helix-loop-helix transcription factor known to regulate embryonic hematopoiesis. This transcription factor binds as a heterodimer with E2A and HEB/HTF4 to a nucleotide sequence motif termed the E-box. In addition, leukemogenesis is accelerated dramatically by transgenic co-expression of TAL1 and the catalytic subunit of casein kinase IIalpha, a serine/ threonine protein kinase known to modulate the activity of other bHLH transcription factors.

Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.