Tubulin beta (Tubulin beta-3 Chain, Tubulin beta-4 Chain, Tubulin beta-III, TUBB3, TUBB4) (HRP)

Antibodies / Antibodies-Tubulin

-20°C

Supplied as a liquid in PBS, pH 7.2, 50% glycerol. Labeled with horseradish peroxidase (HRP).

Synthetic peptide corresponding to amino acids 402-455 of Human Tubulin beta.

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55, 000 Da.
Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10, 000
Immunohistochemistry: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.