U2AF2 (hU2AF(65),hU2AF65,Splicing Factor U2AF 65kD Subunit,U2 auxiliary Factor 65kD Subunit,U2 Small Nuclear Ribonucleoprotein auxiliary Factor (65kD),U2 Small Nuclear RNA auxiliary Factor 2,U2 snRNP auxiliary Facto

Antibodies / Antibodies-Splicing Factors

-20°C

Supplied as a liquid PBS,  0.1% sodium azide,  50% glycerol.

Recombinant full length Human U2AF2.

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.