alpha-Tubulin (Tubulin alpha-1A chain)

Pab

Blue Ice

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Recombinant protein corresponding to alpha-tubulin.

Tubulin is a major cytoskeleton component that has three distinct forms, designated alpha, beta and y Tubulin. alpha and beta Tubulins form heterodimers, which multimerize to form a microtubule filament. y Tubulin forms a soluble multiprotein particle with several other proteins. This particle, designated the gammasome, is required for nucleating microtubule filaments at the centrosome. In several organisms, numerous isoforms of the Tubulins exist that are encoded by different genes. The alpha and beta isoforms undergo a variety of post-translational modifications, which may affect microtubule stability and protein interactions. High expression of class II beta Tubulin has been seen in elongating axons, indicating a role in neurite outgrowth. Tubulins may also play a role in non-neuronal cell process formation.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.