MECP2 (AUTSX 3,AUTSX3,DKFZp686A24160,Mbd 5,Mbd5,MECP 2,MeCP 2 Protein,MeCP-2 Protein,MeCP2,Mecp2,MECP2_HUMAN,Methyl CpG Binding Protein 2 (Rett Syndrome),Methyl CpG Binding Protein 2,Methyl-CpG-Binding Protein 2,MRX 16,M

Antibodies / Antibodies-Binding Proteins

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.

Fusion protein of human MECP2

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:2000
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:100-1:300
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.