Carcinoembryonic Antigen (CEA, CD66e) (MaxLight 550)

Mab

MaxLight 550

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.

Human CD66e

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150, 000.

Applications:
Suitable for use in Flow Cytometry, FLISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen/paraffin): 1:1000, requires protein digestion pre-treatment of paraffin sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3NSI myeloma cell line.

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.