CD68 (Macrosialin, Macrophage Antigen, SCARD1, Scavenger Receptor Class D Member1)

Antibodies / Antibodies-Receptors, Scavenger

-20°C

Supplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.

Fusion protein corresponding to external domain of human CD68.

Recognizes CD68 which is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. Reacts with myeloid precursors, peripheral blood granulocytes and plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. Shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.

Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Not recommended for use in Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin): 1:40-1:80. Requires antigen retrieval using heat treatment prior to staining of paraffin sections; 1mM EDTA, pH 8.0, is recommended.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
p3-NS1-Ag4-1 myeloma cells with spleen cells from Balb/c mice.

Positive Control:
Human Tonsil

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb x Mo

Recommended Negative Control:
I1904-84Q3 IgG2b Murine Negative Control

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.