eIF2C1 (Eukaryotic Translation Initiation Factor 2C1, eIF2C 1, eIF-2C 1, Argonaute 1, AGO1, hAgo1, Golgi Endoplasmic Reticulum Protein 95kD, GERP95, Protein Argonaute-1, Putative RNA-binding Protein Q99)

Antibodies / Antibodies-Translation Initiation Factors

-20°C

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Synthetic peptide corresponding to the sequence around Ile392 of human Argonaute 1 protein.

Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1, 2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4) and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are
about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation and stress response (1, 2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1, 2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1, 2). Argonaute proteins participate in the various steps of
microRNA-mediated gene silencing such as repression of translation and mRNA turnover (1).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.