Sialoprotein, Bone (BSP, Bone Sialoprotein 2, BSP2, Cell-binding Sialoprotein, Integrin-binding Sialoprotein)

Antibodies / Antibodies-Extracellular Matrix Proteins

-20°C

Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile dH2O.

Human Bone Sialoprotein (bone extract)

Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling.

Applications:
Suitable for use in ELISA, Western Blot, RIA, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
ELISA: 1ug/ml
Western Blot: 1:100
Immunohistochemistry (Paraffin): 4ug/ml
RIA: 20ng/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.