ALDH2 Antibody

P05091

1.Eur Heart J. 2012 Jul; 33(13):1606-14. 2.Clin Toxicol (Phila). 2012 Apr; 50(4):242-9.

217

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);

1/10000

WB

Figure 2: Western blot analysis using ALDH2 mAb against HEK293 (1) and ALDH2 (AA: 317-517)-hIgGFc transfected HEK293 (2) cell lysate.

1/500 - 1/2000

WB

Figure 4: Immunofluorescence analysis of HepG2 cells using ALDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

1/75

FC

Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALDH2 mouse mAb with DAB staining.

1/200 - 1/1000

56381

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Purified recombinant fragment of human ALDH2 (AA: 317-517) expressed in E. Coli.