Comparison

ALDH2 Antibody

Item no. ABC-AO2295a
Manufacturer Abcepta
Amount 100 ul
Category
Type Antibody Primary
Applications WB, FC, IHC, ICC, ELISA
Clone 4G6A3
Specific against other
Host Mouse
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products ALDH2, ALDM, Aldehyde dehydrogenase, mitochondrial, ALDH class 2, ALDH-E2, ALDHI, 1.2.1.3
Available
Primary Accession
P05091
Application
WB, IHC, FC, ICC, E
Bio References
1.Eur Heart J. 2012 Jul; 33(13):1606-14. 2.Clin Toxicol (Phila). 2012 Apr; 50(4):242-9.
Clonality
Monoclonal
Gene ID
217
Gene Name
ALDH2
Subtitle
Purified Mouse Monoclonal Antibody
Reactivity
H, M, Rat
Legend image 1

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);

Type image 1
E
Dilution image 1
1/10000
Legend image 2
Figure 1: Western blot analysis using ALDH2 mAb against human ALDH2 recombinant protein. (Expected MW is 47.4 kDa)
Type image 2
WB
Dilution image 2
1/500 - 1/2000
Legend image 3
Figure 2: Western blot analysis using ALDH2 mAb against HEK293 (1) and ALDH2 (AA: 317-517)-hIgGFc transfected HEK293 (2) cell lysate.
Type image 3
WB
Dilution image 3
1/500 - 1/2000
Legend image 4
Figure 3: Western blot analysis using ALDH2 mouse mAb against HepG2 (1), A549 (2) cell lysate, and Rat live (3), Mouse liver (4), Mouse brain (5) tissue lysate.
Type image 4
WB
Dilution image 4
1/500 - 1/2000
Legend image 5
Figure 4: Immunofluorescence analysis of HepG2 cells using ALDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Type image 5
IF
Dilution image 5
1/75
Legend image 6
Figure 5: Flow cytometric analysis of HeLa cells using ALDH2 mouse mAb (green) and negative control (red).
Type image 6
FC
Dilution image 6
1/200 - 1/400
Legend image 7
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALDH2 mouse mAb with DAB staining.
Type image 7
IHC
Dilution image 7
1/200 - 1/1000
Isotype
IgG1
Calculated MW
56381
Formulation
Purified antibody in PBS with 0.05% sodium azide
Description
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Clone: 4G6A3
Immunogen
Purified recombinant fragment of human ALDH2 (AA: 317-517) expressed in E. Coli.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ul
Available: In stock
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