Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB, FC |
Menge |
80 ul |
Host |
Rabbit |
ArtNr |
ABC-AP10973c-ev |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
Q9NZJ0 |
Antigen Type |
Synthetic Peptide |
Application |
FC, WB |
Bio Background |
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. |
Bio References |
Centore, R.C., et al. Mol. Cell 40(1):22-33(2010)Abbas, T., et al. Mol. Cell 40(1):9-21(2010)Song, B., et al. Mol. Cancer 9, 96 (2010) :Li, J., et al. Br. J. Cancer 101(4):691-698(2009)Abbas, T., et al. Genes Dev. 22(18):2496-2506(2008) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB24420 |
Gene ID |
51514 |
Gene Name |
DTL |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
DTL Antibody (Center) (Cat. #AP10973c) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the DTL antibody detected the DTL protein (arrow). |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
DTL Antibody (Center) (Cat. #AP10973c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 2 |
FC |
Dilution image 2 |
1:10~50 |
Other Accession |
Q3TLR7; NP_057532.2 |
Isotype |
Rabbit Ig |
Calculated MW |
79468 |
Antigen Region |
229-256 |
Antigen Source |
HUMAN |
Target/Specificity |
This DTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of human DTL. |
NCBI Accession |
NP_057532.2 |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.