« Back
Home /
DTL Antibody (Center)

DTL Antibody (Center)

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC
Amount	80 ul
Host	Rabbit
Item no.	ABC-AP10973c-ev
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q9NZJ0
Antigen Type	Synthetic Peptide
Application	FC, WB
Bio Background	Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
Bio References	Centore, R.C., et al. Mol. Cell 40(1):22-33(2010) Abbas, T., et al. Mol. Cell 40(1):9-21(2010) Song, B., et al. Mol. Cancer 9, 96 (2010) : Li, J., et al. Br. J. Cancer 101(4):691-698(2009) Abbas, T., et al. Genes Dev. 22(18):2496-2506(2008)
Clonality	Polyclonal
Clone/Animal Names	RB24420
Gene ID	51514
Gene Name	DTL
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	DTL Antibody (Center) (Cat. #AP10973c) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the DTL antibody detected the DTL protein (arrow).
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	DTL Antibody (Center) (Cat. #AP10973c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 2	FC
Dilution image 2	1:10~50
Other Accession	Q3TLR7; NP_057532.2
Isotype	Rabbit Ig
Calculated MW	79468
Antigen Region	229-256
Antigen Source	HUMAN
Target/Specificity	This DTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of human DTL.
NCBI Accession	NP_057532.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-AP10973c-ev-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 80 ul

available

Delivery expected until 6/6/2024