Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, IHC-P |
Menge |
400 ul |
Host |
Rabbit |
ArtNr |
ABC-AP2800a |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
Q01130 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P |
Bio Background |
SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway. |
Bio References |
Merdzhanova, G., Cell Death Differ. 15 (12), 1815-1823 (2008) Solis, A.S., J. Biol. Chem. 283 (35), 23619-23626 (2008) Donev, R., Mol. Psychiatry 12 (7), 681-690 (2007) Sureau, A., Proc. Natl. Acad. Sci. U.S.A. 89 (24), 11683-11687 (1992) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB17329 |
Gene ID |
6427 |
Gene Name |
SRSF2 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-SFRS2 Antibody (N-term) at 1:4000 dilutionLane 1: HepG2 whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:4000 |
Legend image 2 |
Western blot analysis of anti-SFRS2 Antibody (N-term) (Cat.#AP2800a) in K562 cell line lysates (35ug/lane).SFRS2(arrow) was detected using the purified Pab. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SFRS2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Other Accession |
Q6PDU1; Q06A98; Q62093; P30352; Q3MHR5 |
Predicted |
B, C, M, Pig, Rat |
Isotype |
Rabbit Ig |
Calculated MW |
25476 |
Antigen Region |
9-39 |
Antigen Source |
HUMAN |
Target/Specificity |
This SFRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human SFRS2. |
NCBI Accession |
NP_001182356.1; NP_003007.2 |
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