SFRS2 Antibody (N-term)

Synthetic Peptide

SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.

Polyclonal

6427

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-SFRS2 Antibody (N-term) at 1:4000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: K562 whole cell lysate


Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 25 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:4000

WB

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SFRS2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

1:50~100

B, C, M, Pig, Rat

25476

HUMAN

NP_001182356.1; NP_003007.2