Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB, IHC-P, Dot |
Menge |
400 ul |
Host |
Rabbit |
ArtNr |
ABC-AP3159a |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
Q02750 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, DB, WB |
Bio Background |
MEK1is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax. |
Bio References |
Ohren, J.F., et al., J. Neural Transm. 11(12):1192-1197 (2004). Naegele, S., et al., J. Biol. Chem. 279(44):46023-46034 (2004). Ussar, S., et al., J. Biol. Chem. 279(42):43861-43869 (2004). Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004). Spence, H.J., et al., EMBO Rep. 5(5):484-489 (2004). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB05985 |
Gene ID |
5604 |
Gene Name |
|
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of lysates from Hela cell line, untreated or treated with TPA(200nM, 30min), using Bi-Phospho-MEK1(S218/222) Antibody(upper) or GAPDH (lower). |
Type image 1 |
WB |
Dilution image 1 |
1:500 |
Legend image 2 |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:50~100 |
Legend image 3 |
Dot blot analysis of anti-Phospho-MEK1 Pab (Cat. #AP3159a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
Type image 3 |
DB |
Dilution image 3 |
1:500 |
Other Accession |
P36506; Q63932; P36507; Q90891; Q05116; Q01986; P29678; P31938; Q63980; Q10664; Q24324 |
Predicted |
D, E, Ha, M, Rb, Rat, X, C |
Isotype |
Rabbit Ig |
Calculated MW |
43439 |
Antigen Source |
HUMAN |
Target/Specificity |
This MEK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S218/222 of human MEK1. |
NCBI Accession |
NP_002746.1 |
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