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Bi-Phospho-MEK1(S218/222) Antibody

Bi-Phospho-MEK1(S218/222) Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Isotype	Ig
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IHC-P, Dot
Amount	400 ul
Host	Rabbit
Item no.	ABC-AP3159a
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q02750
Antigen Type	Synthetic Peptide
Application	IHC-P, DB, WB
Bio Background	MEK1is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Bio References	Ohren, J.F., et al., J. Neural Transm. 11(12):1192-1197 (2004). Naegele, S., et al., J. Biol. Chem. 279(44):46023-46034 (2004). Ussar, S., et al., J. Biol. Chem. 279(42):43861-43869 (2004). Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004). Spence, H.J., et al., EMBO Rep. 5(5):484-489 (2004).
Clonality	Polyclonal
Clone/Animal Names	RB05985
Gene ID	5604
Gene Name	MAP2K1 (HGNC:6840)
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of lysates from Hela cell line, untreated or treated with TPA(200nM, 30min), using Bi-Phospho-MEK1(S218/222) Antibody(upper) or GAPDH (lower).
Type image 1	WB
Dilution image 1	1:500
Legend image 2	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 2	IHC-P
Dilution image 2	1:50~100
Legend image 3	Dot blot analysis of anti-Phospho-MEK1 Pab (Cat. #AP3159a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Type image 3	DB
Dilution image 3	1:500
Other Accession	P36506; Q63932; P36507; Q90891; Q05116; Q01986; P29678; P31938; Q63980; Q10664; Q24324
Predicted	D, E, Ha, M, Rb, Rat, X, C
Isotype	Rabbit Ig
Calculated MW	43439
Antigen Source	HUMAN
Target/Specificity	This MEK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S218/222 of human MEK1.
NCBI Accession	NP_002746.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 7/4/2024