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Phospho-STAT1(S727) Antibody

Phospho-STAT1(S727) Antibody

ArtNr	ABC-AP3256a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	STAT1, Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
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Primary Accession	P42224
Antigen Type	Synthetic Peptide
Application	WB, IHC-P
Bio Background	In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.
Bio References	Garcin, D., et al., J. Virol. 78(16):8799-8811 (2004). Melen, K., et al., J. Med. Virol. 73(4):536-547 (2004). Klampfer, L., et al., J. Biol. Chem. 279(29):30358-30368 (2004). Marg, A., et al., J. Cell Biol. 165(6):823-833 (2004). Chim, C.S., et al., Blood 103(12):4630-4635 (2004).
Clonality	Polyclonal
Clone/Animal Names	RB6033
Gene ID	6772
Gene Name	STAT1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of anti-Phospho-STAT1 Pab (Cat. #AP3256a) in mouse brain tissue lysate (35ug/lane). Phospho-STAT1(arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 2	IHC-P
Dilution image 2	1:50~100
Other Accession	Q764M5; P42225
Predicted	M, Pig
Isotype	Rabbit Ig
Calculated MW	87335
Antigen Source	HUMAN
Target/Specificity	This STAT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT1.
NCBI Accession	NP_009330.1; NP_644671.1

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Menge: 400 ul

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