MMP14 Antibody (N-term)

Synthetic Peptide

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head and neck.

Polyclonal

4323

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-MMP14 Antibody (N-term) at 1:2000 dilution
Lane 1: A2058 whole cell lysate
Lane 2: HT-1080 whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lane 4: NCI-H292 whole cell lysate
Lane 5: Mouse kidney lysate
Lane 5: Rat kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 66 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

AP6198a staining MMP14 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

1:25

FC

Q10739; Q9XT90; P53690; Q9GLE4; NP_004986

Rabbit Ig

145-174

This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of human MMP14.