Vergleich

KRT10 Antibody (N-term)

ArtNr ABC-AP6704a
Hersteller Abcepta
Menge 400 ul
Kategorie
Typ Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen WB, FC, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products Keratin, KRT10, CK-10, K10, KPP, type I cytoskeletal 10, Cytokeratin-10, Keratin-10
Lieferbar
Primary Accession
P13645
Antigen Type
Synthetic Peptide
Application
FC, IHC-P, WB
Bio Background
KRT10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in its gene are associated with epidermolytic hyperkeratosis.
Bio References
Morais, P., Eur J Dermatol 19 (4), 333-336 (2009)
Barcelos, A.C., J. Cutan. Pathol. 36 (6), 647-654 (2009)
Clonality
Polyclonal
Clone/Animal Names
RB18412
Gene ID
3858
Gene Name
KRT10
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Anti-KRT10 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 59 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1
WB
Dilution image 1
1:2000
Legend image 2
Formalin-fixed and paraffin-embedded human kidney carcinoma with KRT10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2
IHC-P
Dilution image 2
1:50~100
Legend image 3
Flow cytometric analysis of 293 cells using KRT10 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3
FC
Dilution image 3
1:10~50
Other Accession
P02535
Predicted
M
Isotype
Rabbit Ig
Calculated MW
58827
Antigen Region
150-179
Antigen Source
HUMAN
Target/Specificity
This KRT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the N-terminal region of human KRT10.
NCBI Accession
NP_000412.3

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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