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Pyruvate Kinase (PKM2) Antibody (C-term)

Pyruvate Kinase (PKM2) Antibody (C-term)

ArtNr	ABC-AP7044B
Hersteller	Abcepta
Menge	200 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PKM2, PKM, OIP3, PK2, PK3, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Tumor M2-PK, p58, CTHBP, THBP1, Pyruvate kinase PKM, OIP-3
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Primary Accession	P14618
Antigen Type	Synthetic Peptide
Application	WB, IHC-P, IF
Bio Background	There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
Bio References	References for protein: 1.Williams, J.M., et al., Mol. Microbiol. 27(1):171-186 (1998). 2.Gress, T.M., et al., Oncogene 13(8):1819-1830 (1996). 3.Kato, H., et al., Proc. Natl. Acad. Sci. U.S.A. 86(20):7861-7865 (1989). 4.Tsutsumi, H., et al., Genomics 2(1):86-89 (1988). 5.Tani, K., et al., Gene 73(2):509-516 (1988). References for MCF7 cell line: 1.Soule, HD; Vazquez J; Long A; Albert S; Brennan M. (1973). "A human cell line from a pleural effusion derived from a breast carcinoma". Journal of the National Cancer Institute 51 (5): 1409–1416. [PMID 4357757]. 2.Levenson, AS; Jordan VC. (1997). "MCF-7: the first hormone-responsive breast cancer cell line". Cancer Research 57 (15): 3071–3078. [PMID 9242427]. 3.Lacroix, M; Leclercq G. (2004). "Relevance of breast cancer cell lines as models for breast tumours: an update". Breast Research and Treatment 83 (3): 249–289.[PMID 14758095].
Clonality	Polyclonal
Clone/Animal Names	RB3697
Gene ID	5315
Gene Name	PKM
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Pr, Rat
Legend image 1	Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) (C-term) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP7044b Pyruvate Kinase (PKM2) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).
Type image 1	IF
Dilution image 1	1:200
Legend image 2	All lanes : Anti-PKM2 Antibody (N491) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of hPKM2-N491 (Cat.# AP7173a) in A2058 cell line(lane 1) and mouse brain tissue(lane 2) lysates (35ug/lane). PKM2 (arrow) was detected using the purified Pab.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	All lanes : Anti-hPKM2-N491 at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: C2C12 whole cell lysate Lane 5: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 4	WB
Dilution image 4	1:2000
Legend image 5	Pyruvate Kinase (PKM2) Antibody (C-term) (Cat. #AP7044b) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 5	IHC-P
Dilution image 5	1:50~1:100
Other Accession	P14786
Isotype	Rabbit Ig
Calculated MW	57937
Antigen Region	476-505
Antigen Source	HUMAN
Target/Specificity	This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
NCBI Accession	NP_001193725.1; NP_001193726.1; NP_001193727.1; NP_001193728.1; NP_002645.3; NP_872270.1; NP_872271.1

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