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AMPK alpha (PRKAA1) Antibody (S487)

AMPK alpha (PRKAA1) Antibody (S487)

ArtNr	ABC-AP7260a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, Tau-protein kinase PRKAA1, AMPK subunit alpha-1, ACACA kinase, HMGCR kinase
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Primary Accession	Q13131
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Bio References	Pang, T., J. Biol. Chem. 282 (1), 495-506 (2007) Crawford, R.M., Cell. Signal. 18 (10), 1595-1603 (2006) Crawford, R.M., Mol. Cell. Biol. 26 (15), 5921-5931 (2006)
Clonality	Polyclonal
Clone/Animal Names	RB13341
Gene ID	5562
Gene Name	PRKAA1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-PRKAA1 Antibody (S487) at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Western blot analysis of PRKAA1 (arrow) using rabbit polyclonal PRKAA1 Antibody (S246) (Cat.#AP7260a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (Lane 2) (Origene Technologies).
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PRKAA1-pS487, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Other Accession	Q86VS1
Isotype	Rabbit Ig
Calculated MW	64009
Antigen Region	474-502
Antigen Source	HUMAN
Target/Specificity	This AMPK alpha(PRKAA1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-502 amino acids from human AMPK alpha(PRKAA1).
NCBI Accession	NP_006242.5; NP_996790.3

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Menge: 400 ul

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Lieferung vsl. bis 06.11.2025

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