Caspase-3 (CASP3) Antibody (Center)

Synthetic Peptide

CASP3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Polyclonal

836

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-CASP3 Antibody (Center) at 1:2000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: A431 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 32 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody (Center) (Cat.#AP7563c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.

1:1000

IHC-P

Flow cytometric analysis of NCI-H460 cells using Caspase-3 (CASP3) Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

31608

HUMAN

NP_004337.2; NP_116786.1