ROR1 Antibody

Recombinant Protein

ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.

Polyclonal

4919

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-ROR1 Antibody at 1:4000 dilution
Lane 1: K562 whole cell lysate
Lane 2: MDA-MB-231 whole cell lysate
Lane 3: NCI-H226 whole cell lysate
Lane 4: A549 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lane 6: Mouse kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 104 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:4000

WB

Immunohistochemical analysis of AP7671d on paraffin-embedded Human heart tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

1:100

IHC-P

Overlay histogram showing A549 cells stained with AP7671d(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP7671d, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

104283

This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).