Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC, IHC-P |
Amount |
200 ul |
Host |
Rabbit |
Item no. |
ABC-AP7671D |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
Q01973 |
Antigen Type |
Recombinant Protein |
Application |
IHC-P, WB, FC |
Bio Background |
ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development. |
Bio References |
Nomi, M., et al., Mol. Cell. Biol. 21(24):8329-8335 (2001).Reddy, U.R., et al., Genomics 41(2):283-285 (1997).Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996).Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB14753 |
Gene ID |
4919 |
Gene Name |
ROR1 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
All lanes : Anti-ROR1 Antibody at 1:4000 dilutionLane 1: K562 whole cell lysateLane 2: MDA-MB-231 whole cell lysateLane 3: NCI-H226 whole cell lysateLane 4: A549 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: Mouse kidney tissue lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:4000 |
Legend image 2 |
Anti-ROR1 Antibody at 1:4000 dilution + PANC-1 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:4000 |
Legend image 3 |
Immunohistochemical analysis of AP7671d on paraffin-embedded Human heart tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:100 |
Legend image 4 |
Immunohistochemical analysis of AP7671d on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
Type image 4 |
IHC-P |
Dilution image 4 |
1:100 |
Legend image 5 |
Overlay histogram showing A549 cells stained with AP7671d(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP7671d, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Type image 5 |
FC |
Dilution image 5 |
1:25 |
Isotype |
Rabbit Ig |
Calculated MW |
104283 |
Antigen Source |
HUMAN |
Target/Specificity |
This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399). |
NCBI Accession |
NP_001077061.1; NP_005003.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.