IRS2 Antibody (C-term)

Synthetic Peptide

Insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. This protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

Polyclonal

8660

Purified Rabbit Polyclonal Antibody (Pab)

Fluorescent image of Hela cell stained with IRS2 Antibody (C-term)(Cat#AP7789b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).IRS2 immunoreactivity is localized to Cytoplasm significantly.

1:10~50

WB

Western blot analysis of anti-IRS2 Antibody (C-term) (Cat.#AP7789b) in 293 cell line lysates (35ug/lane). IRS2 (arrow) was detected using the purified Pab.

1:1000

IHC-P

Rabbit Ig

1309-1338

This IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2.