BRAF Antibody (T400)

ArtNr	ABC-AP7810E
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	BRAF, p94, BRAF1, RAFB1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1
Lieferbar
Primary Accession	P15056
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Bio References	Hingorani, S.R., et al., Cancer Res. 63(17):5198-5202 (2003). Lee, J.W., et al., Br. J. Cancer 89(10):1958-1960 (2003). Davies, H., et al., Nature 417(6892):949-954 (2002). Naoki, K., et al., Cancer Res. 62(23):7001-7003 (2002). Stephens, R.M., et al., Mol. Cell. Biol. 12(9):3733-3742 (1992).
Clonality	Polyclonal
Clone/Animal Names	RB13721
Gene ID	673
Gene Name	BRAF (HGNC:1097)
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-BRAF Antibody (T400) at 1:500-2000 dilution Lane 1: K562 whole cell lysate Lane 2: Raji whole cell lysate Lane 3: T47D whole cell lysate Lane 4: A20 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:500-2000
Legend image 2	Anti-BRAF Antibody (T400) at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:500
Legend image 3	Immunohistochemical analysis of AP7810E on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:100
Legend image 4	Immunohistochemical analysis of AP7810E on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 4	IHC-P
Dilution image 4	1:100
Other Accession	P28028
Predicted	M
Isotype	Rabbit Ig
Calculated MW	84437
Antigen Region	379-408
Antigen Source	HUMAN
Target/Specificity	This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 amino acids from human BRAF.
NCBI Accession	NP_004324.2

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